Figure 4.

In vivo suppression of Tconv by antigen-specific iTreg cells. FIR mice were adoptively transferred with a mixed population of OT-I and OT-II cells, together with either OT-II iTreg, polyclonal iTreg or nTreg. 5 days following immunization with ova/alum, the absolute numbers of OT-I and OT-II Tconv cells were analyzed in the mLN (A) and spleen (B). Data are shown for individual mice together with mean ± S.E.M. Expression of CD11a, CD80 and CD86 were analyzed on CD11c positive peritoneal cells on day 5 after immunization and representative plots are shown (C).