Figure 4. Blocking both the STAT3 and the p38 MAPK pathway improves the T cell stimulatory functions of human MoDC differentiated in the presence of U373 glioma supernatant. (A) Capacity of DC differentiated in the presence of U373 supernatant with or without the STAT3 and/or p38 MAPK inhibitors to stimulate allogeneic T-cell proliferation, means ± sd are shown, n = 4; *p < 0.05 between indicated conditions. Photographic inserts show proliferating T cell aggregates for the different conditions (magnification 400x). (B) Secretion levels of IL-12p70 and IL-10 by MoDC differentiated in the presence or absence of U373 supernatant with or without the STAT3 and/or p38 MAPK inhibition; expressed in pg/ml or as IL-12p70:IL-10 ratios. Shown are mean ± SD from 4 experiments (C) Secretion of Th1/Th2/Th17 cytokines at day four in the MLR cultures described under (A). Shown are mean ± SD from four experiments; *p < 0.05 between the indicated conditions.