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. 1976 Apr;3(4):953–963. doi: 10.1093/nar/3.4.953

Isolation of single stranded DNA related to the transcriptional activity of animal cells.

H Tapiero, S A Leibowitch, D Shaool, M N Monier, J Harel
PMCID: PMC342957  PMID: 944919

Abstract

Single stranded DNA (s.s.DNA) comprising 1-2% of the total nuclear DNA was isolated by an improved method of hydroxyapatite chromatography from native nuclear DNA3 of embryonic chick cells, labeled for several cell generations with 3H-thymidine. Small quantities of 3H-DNA were annealed with a large excess of unlabeled DNA or polysomal RNA from chick embryos. Hybridization kinetics (monitored by the use of SI nuclease digestion, hydroxyapatite chromatography and thermalfusion), indicated that s.s.DNA belongs to the non repetitious fraction of the cell genome. One third represents DNA sequences engaged in the transcription of messenger RNA's.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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