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. 2012 Aug 1;1(5):670–686. doi: 10.4161/onci.20426

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Figure 4.T-cell reactivity against multiple unrelated hTERT Th and CTL epitopes. (A) shows pentamer analysis of hTERT-specific HLA-B*0702 restricted CD8+ T cells post vaccination. PBMCs were stained ex vivo with two pentamers with novel hTERT peptides 613–621 and 672–681 which were nested epitopes of GV1001 and 660–689, respectively. Vaccination started in 2002 and post vaccination samples from the years indicated were tested. Black bars represent T cells specific for peptide 613–621-specific, dotted bars peptide 672–681 and hatched bars the HIV control peptide. (B) shows a summary of all post-vaccination-T cell responses detected against several unrelated 15- and 30-mer hTERT peptides. PBMCs from different post-vaccination sample time points were pre-stimulated with 25 overlapping hTERT peptides and tested for proliferation against single peptides.