Figure 7. HLA-DR*0801 restricted CD4+ T cell clone specific for novel hTERT epitope recognizes melanoma cell line. Intracellular cytokine staining of the hTERT-specific HLA-DR*08 restricted CD4+ T cell clone 109 was performed after overnight stimulation with autologus EBV-LCL or the HLA-DR*0801 melanoma cell line, ESTDAB-100. As positive controls, the EBV-LCL and ESTDAB-100 were both loaded with hTERT 30-mer peptide 660–689.