Figure 4. Analysis of iCLIP crosslink clusters around the exons regulated by FUS.

The map of crosslink clusters at positions within 500 nt of alternative exons and flanking exons. Crosslink clusters were determined using flank = 200 and FDR<0.05. The exons are grouped by sequential analysis of crosslink cluster positions in three regions: group 1 is identified by clusters within the exon; group 2 by clusters downstream of the exon; and group 3 by clusters upstream of the exon. The cassette exons with ΔIrank > 1 (enhanced exons, red clusters) or ΔIrank < −1 (repressed exons, blue clusters) in analysis of FUS^−/− brain RNA that contain at least one crosslink cluster in these regions are shown. The positions of FUS (a), TDP-43 (b) and U2AF65 (c) crosslink clusters are shown, and the gene symbol and exon number (according to genomic annotation) is shown on the left. Only the FUS-regulated exons that contain crosslink clusters for the corresponding RBP in the evaluated regions are shown.