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Gel analysis of a-factor biosynthetic intermediates. The precursor (P0, P1, and P2) and mature (M) forms of a-factor are resolved on a 16% SDS-polyacrylamide gel, as discussed in the text (see also Fig. 3). Cells were pulse-labeled for 5 min with Tran35S-label, intracellular (I) and extracellular (E) fractions prepared, and proteins immunoprecipitated with a-factor antibodies. (Reprinted from reference 56.)
