Figure 5. Size of polyplexes released from PLGA MPs over time.
Although the average diameters of the polyplexes for each siRNA type were similar when considering all timepoints together (135 ± 50 nm for siRNA1-PEI and 117 ± 50 nm for siRNA2-PEI), the size of the two polyplex types at each timepoint differed significantly (p < 0.05), based on repeated measures analysis of variance. Data are presented as the mean ± standard deviation for (n = 4) samples at each timepoint. Error bars are included for all data points, though they are too small to resolve in some cases. The white-filled data points at 0 nm correspond to groups with no detectable polyplexes (e.g., only free PEI was detected for siRNA2-PEI on day 3, as shown in Figure 6).