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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Plant J. 2012 Jun 14;71(5):776–786. doi: 10.1111/j.1365-313X.2012.05028.x

Figure 4. Mutations in histone H1 affect FWA and FIS2 imprinting in Arabidopsis endosperm.

Figure 4

(a) The maternal histone h1 mutation diminishes maternal FWA expression in endosperm.

(b) The maternal histone h1 mutation reduces maternal FIS2 expression in endosperm.

Endosperm tissues from seed at 8-DAP of respective crossed siliques were collected and total RNA was isolated. All h1 mutants are in Col-0 background and the maternal parent was shown first in the crosses (♀ X ♂). The FWA and FIS2 alleles from Col-0 and Ler were distinguished as described (Kinoshita et al., 2004; Jullien et al., 2006a). FWA and FIS2 expression was examined by using semi-quantitative RT-PCR. ACTIN (ACT) was amplified from the cDNA as a control.