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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Am J Med Genet A. 2012 Aug 7;158A(9):2204–2213. doi: 10.1002/ajmg.a.35532

FIG. 2.

FIG. 2

Hypersensitivity of Smarcal1del/del mice to CPT-11. a-d: Photomicrographs of TUNEL-positive nuclei in splenic tissue from postnatal day (P) 36 Smarcal1del/del and Smarcal1+/+ mice following 5 daily ip-injections with PBS. Panels c and d are higher magnification photomicrographs of the boxed areas in panels a and b, respectively. e: Graph showing the average weight gain of Smarcal1del/del (n = 4) and Smarcal1+/+ (n = 4) mice receiving daily ip-injections of PBS from P28 through P84 and during 90 days following treatment (shaded area). f: Representative skeletal radiographs of Smarcal1del/del and Smarcal1+/+ mice at P174 following 8 weeks of daily injections with PBS. g-j: Photomicrographs of TUNEL-positive nuclei in splenic tissue from P36 Smarcal1del/del and Smarcal1+/+ mice following 5 daily ip-injections with CPT-11. Panels i and j are higher magnification photomicrographs of the boxed areas in panels g and h, respectively. k: Graph showing the average weight gain of Smarcal1del/del (n = 3) and Smarcal1+/+ (n = 4) mice receiving daily ip-injections of CPT-11 from P28 through P84 and during 90 days following treatment (shaded area). l: Representative skeletal radiographs of Smarcal1del/del and Smarcal1+/+ mice at P173 following 8 weeks of daily injections with CPT-11. m-t: Photomicrographs of representative sections of the distal femoral growth plate of Smarcal1+/+ and Smarcal1del/del male mice treated with PBS or CPT-11 for 8 weeks. The tissue was stained with H&E. Panels q-t are higher magnification photomicrographs of the boxed areas in panels m-p, respectively. Note the poorly organized columns of chondrocytes in the smaller hypocellular growth plates of the CPT-11 treated Smarcal1del/del mice. u: Graph showing the mean bone density of the distal femur of Smarcal1+/+ and Smarcal1del/del mice treated either with PBS or CPT-11. Scale bars = 100 μm. Error bars represent one standard deviation. For statistically significant differences between Smarcal1del/del and Smarcal1+/+ mice, a * indicates P<0.05 and a ** indicates P<0.01. Abbreviations: CPT-11, irinotecan; ip, intraperitoneal; mg HA/ccm, milligrams hydroxyapatite per cubic centimetre.