Table 2.
QS regulator expression in KLH11 null mutants
| β-Galactosidase Sp. Act.1 | |||||
|---|---|---|---|---|---|
| KLH11 Extracts |
Synthetic AHLs |
||||
| Mutants2 | Relevant Genotype |
No Extract | 2.5% | No AHL | + AHL3 |
| EC2 | ssaI-lacZ, null | 6 (1.7) | 95 (10.6) | 6.3 (0.7) | 213.3 (9.2) |
| EC3 | ssbI-lacZ, null | 1 (<1) | <1 (<0.1) | 1.1 (0.3) | 1.1(<0.1) |
| JZ1 | ssaR-lacZ, WT | 107.1(0.8) | 105.0 (4.4) | 93.7 (8.3) | 99.3 (2.8) |
| EC4 | ssaR-lacZ, null | 23 (3) | 19 (1.4) | 26.7 (1.3) | 28.6 (2.4) |
| JZ2 | ssbR-lacZ, WT | 11.6 (2.8) | 10.8 (3.1) | 12.0 (0.5) | 12.6 (0.5) |
| EC5 | ssbR-lacZ, null | 15 (<) | 10 (4.6) | 27.6 (4.5) | 26.0 (4.2) |
1
Specific activity in Miller Units, averages of assays in triplicate (standard deviation)
2
All strains are derived from EC1, spontaneous RifR mutant. Strains EC2, EC3. JZ1 and JZ2 carry Campbell insertions and lacZ transcriptional fusions;
3
2 μM 3-oxo-c16:1 Δ11 was added for ssaIR and 20 μM 3-OH-C14 was added for ssbIR.