Table 3.
Expression of KLH11 PssaI and PssbI promoters in an AHL- host1
| β-Galactosidase Sp. Act.2 | |||
|---|---|---|---|
| Expression plasmid | Fusion plasmid | No AHL | + AHL3 |
| Vector (pBBR1-MCS5) | ssaI-lacZ (pEC116) | 51 (5) | 52 (5) |
| Plac-ssaR (pEC112) | ssaI-lacZ (pEC116) | 341 (15) | 1435 (67) |
| Vector (pBBR1-MCS5) | ssbI-lacZ (pEC121) | <1 (<0.1) | <1 (<0.1) |
| Plac-ssbR (pEC123) | ssbI-lacZ (pEC121) | <1 (<0.1) | <1 (<0.1) |
1
All strains derived from Ti-plasmidless A. tumefaciens NTL4
2
Specific activity in Miller Units, averages of assays in triplicate (standard deviation)
3
2 μM 3-oxo-C16:1 Δ11 was added for ssaI and 20 μM 3-OH-C14 was added for ssbI