Table 2.
Real time PCR primer sequences.
Target gene | Accession No. | Forward | Reverse |
---|---|---|---|
Collagen IIa | NM_001844 | 5′-TGC AGG ATG GGC AGA GGT AT-3′ | 5′-CAC AGA CAC AGA TCC GGC AG-3′ |
Aggrecan | NM_001135 | 5′-TTC TTG GAG AAG GGA GTC CA-3′ | 5′-ACA GCT GCA GTG ATG ACC CT-3′ |
Sox9 | NM_000346 | 5′-GAG GCA GAG GAG GCC ACG GA-3′ | 5′-CCT GGG ATT GCC CCG AGT GC-3′ |
Collagen X | NM_000493 | 5′- CAT AAA AGG CCC ACT ACC CA -3′ | 5′- GTG GAC CAG GAG TAC CTT GC -3′ |
Collagen I | NM_000088 | 5′-AAG AGG AAG GCC AAG TCG AG-3′ | 5′-CAC ACG TCT CGG TCA TGG TA-3′ |
Versican | NM_004385 | 5′-TCT CCC CAG GAA ACT TAC GA-3′ | 5′-CAC TCT TTT GCA GCC TCC TC-3′ |
Osteonectin | NM_003118 | 5′-CTT CAG ACT GCC CGG AGA-3′ | 5′-GAA AGA AGA TCC AGG CCC TC-3′ |
18S rRNA | NM_022551 | 5′-CTT TGC CAT CAC TGC CAT TA-3′ | 5′-ATC CTC AGT GAG TTC TCC CG-3′ |