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Effect of leucine mutations.
A. Sequences of the wild-type, leucine variants and the L16P/P21L mutant. Underlines indicate positions of residues that vary in the leucine variants. Asterisks indicate the position of mutated residues in the L16P/P21L mutant.
B. Growth curves of the mutants in TOP10 cells.
C. (left) Western blot of whole cells for the leucine mutants. An unknown secondary protein product is evident in E-LN. (right) Western blot of membrane fractions.
