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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Mol Microbiol. 2012 Jul 13;85(5):975–985. doi: 10.1111/j.1365-2958.2012.08153.x

Fig. 4.

Fig. 4

Defining the minimal length of E required for lysis activity.

A. Illustration of the lysozyme fusion constructs. e.g. E25 contains the first 25 residues followed by the linker and an inactive lysozyme shown after the arrow.

B. Growth curves of wild-type and the E25–28 fusion constructs in TOP10 cells. Inset, Western blot of whole cells after 10 min of induction.

C. Illustration of the N-terminal deletion constructs.

D. Growth curves of deletion constructs in TOP10 cells. Inset, Western blot of whole cells after 10 min of induction.