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. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Neurosci Methods. 2012 Jun 26;209(2):344–350. doi: 10.1016/j.jneumeth.2012.06.015

Fig. 3. Examples of myelin sheaths stained with FluoroMyelin™ Red.

Fig. 3

Myelinating cultures were incubated for 2 hours in observation medium containing FluoroMyelin™ Red (1:300 dilution) after 34–41 days in culture to visualize the myelin sheaths and then imaged in fresh observation medium using a 40× objective magnification. A–I show examples of 9 different myelin sheaths. Note the smooth appearance of the myelin in these live images. The dye does yield some background staining in these dense cultures, but the staining of the myelin sheaths is much brighter. The inset at the lower right shows an enlarged view of a Schmidt-Lanterman cleft (arrowheads). Scale bar= 25 µm.