Skip to main content
. Author manuscript; available in PMC: 2013 Aug 15.
Published in final edited form as: J Neurosci Methods. 2012 Jun 26;209(2):344–350. doi: 10.1016/j.jneumeth.2012.06.015

Fig. 6. Analysis of cell viability.

Fig. 6

Myelinating cultures after 30–37 days in culture were treated with myelination medium with or without FluoroMyelin™ Red (1:300 dilution) for 2 hours or 24 hours, or they were treated with 0.1% Triton X-100 for 10 minutes to confirm that the assay was capable of detecting dead cells. The percentage of the cells that were alive or dead was determined using Invitrogen’s LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells (see Methods). The data for each treatment are an average of three independent experiments (about 2700 cells in total counted per treatment). On average about 3% of the cells in the untreated cultures were dead, and there was no change after treatment with FluoroMyelin™ Red, even after 24 hours (p>0.3, t-test). Greater than 99% of the cells were scored as dead after treatment with Triton X-100 (positive control).