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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Stroke. 2012 Jun 19;43(9):2423–2429. doi: 10.1161/STROKEAHA.112.656900

Figure 2.

Figure 2

Relationship between SOD1 expression and NSC death in vitro. A, Fluorescent staining of NSCs with HEt (red) and 4′,6 diamidino-2-phenylindole (DAPI) (blue). The increase in HEt signals in the WTNSCs after 8 hours of OGD and 15 minutes of RO was reduced in the TgNSCs, whereas they were enhanced in the KONSCs (n=4). Scale bar, 20 μm. B, NSCs analyzed by TUNEL staining (red) and DAPI (blue) after 8 hours of OGD and 24 hours of RO. The cell-counting study revealed a significant decrease in TUNEL-positivity in the TgNSCs, as well as a significant increase in the KONSCs (n=4). Scale bar, 50 μm. LDH (n=4) (C) and WST-1 (n=4) (D) assays showed a significant reduction in NSC death and increased viability in the TgNSCs after 8 hours of OGD and 24 hours of RO. In contrast, LDH release was increased in the KONSCs compared with the WTNSCs. *P<0.05; †P<0.01; §P<0.001.