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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Stroke. 2012 Jun 19;43(9):2423–2429. doi: 10.1161/STROKEAHA.112.656900

Figure 3.

Figure 3

Reduced grafted-cell death with SOD1 overexpression in vivo. A and C, Fluorescent staining with 4′,6 diamidino-2-phenylindole (DAPI) (blue), GFP (green), and HEt (red) in brain sections 1 hour after transplantation. HEt signals increased in the WTNSCs under ischemic reperfusion injury, but were significantly reduced in the TgNSCs. Conversely, HEt signals were enhanced in the KONSCs compared with the WTNSCs (n=4). The insets represent high magnification images showing colocalization of HEt with GFP-positive grafted cells. MCAO indicates middle cerebral artery occlusion. B and D, Fluorescent staining with DAPI (blue), TUNEL (red), GFP (green), and nestin (magenta) 2 days after transplantation. SOD1 overexpression significantly reduced the number of TUNEL-positive grafted cells in the ischemic brain (n=4). Scale bars, 20 μm. *P<0.05; §P<0.001.