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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2012 May 29;99(0):9–14. doi: 10.1016/j.prostaglandins.2012.05.002

Figure 2. PGE2 promotes the nuclear accumulation of LEF-1.

Figure 2

Panel A. COX-2-silenced cells have reduced levels mRNA levels of LEF-1. Panel B: TPA induces the accumulation of LEF-1 in nuclear cell extracts of COX-2 containing MDA-MB-231 cells, but not in COX-2-silenced derivative Clone 2 cells. Levels of LEF-1 in untreated parental cells are assigned a value of 1. Panel C: Exogenous PGE2 addition induces the accumulation of LEF-1 in nuclear extracts of COX-2-silenced Clone 2 cells. Densitometric evaluation of the expression of LEF-1 levels normalized to the nuclear loading control (Histone 3) is shown for each immunoblot. Levels of LEF-1 in untreated parental cells are assigned a value of 1.