One ovary from postnatal day 3/4 rat was cultured for 2 weeks with either recombinant VEGFA_165, antibodies to VEGFA_XXXB isoforms, an inhibitor of KDR and FLT1 (VEGFA receptors), or an inhibitor to NRP1. The paired ovary from each rat was cultured without treatment to serve as a control. The mean number of follicles at each stage of development was calculated as a percent of total follicles, and these percentages were compared between treated and control ovaries (↑ significant increas in the percent of follicles at each stage in treated ovaries in comparison with controls, ↓ significant decrease in the percent of follicles at each stage in treated ovaries in comparison with controls, - no difference in the percent of follicles at each stage in treated ovaries in comparison with controls). All developing follicles include early primary, primary, transitional, and secondary follicle stages