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Immunogold localization of acrosin in sagittal sections of bull ejaculated sperm, utilizing anti-bull acrosin antibody. a Labeling is confined to the principal (PS) and apical (AS) segments of the acrosome. Little or no labeling is evident in the equatorial segment (ES) of the acrosome nor in the perinuclear theca (PT). b Labeling is closely associated to the IAM. Bars 0.2 μm. See Suppl. Fig. 1a for peptide blocking control
