Observer-independent definition of cytoarchitectonic borders (Schleicher et al., 1999) has been performed in GLI images in cortical regions of interest (A). Dark pixels correspond to high GLI values (high volume fraction of cell bodies), whereas bright pixels correspond to low GLI values (low fraction). GLI profiles were extracted along vertical traverses, and numbered (1 to 129 in this example). Ten features or shape descriptors, based on central moments, were extracted from each profile. Using the multivariate Mahalanobis distance function, borders were defined at the positions where profiles changed significantly in shape. (B) shows a Mahalanobis distance function for neighboring blocks of profiles, each of which contained 14 profiles. Significant distance values were found at positions 16, 45 and 80, marked by arrows in (A) and (B). These positions mark the borders of areas hIP2 and hIP1.