Table A2.

Average silencing efficiency according to categorization of the dinucleotide free energy in separate positions of duplex thermodynamic profile.

Position of dinucleotide from 5′ end	Local duplex stability (kcal/mol)
	−1 ≤ ΔG	2 ≤ ΔG< −1	−3 ≤ ΔG< −2	ΔG< −3
	AVERAGED SILENCING EFFICIENCY PER BIN
1	3.58	1.76	0.55	0.07
2	2	3.03	1.52	0.51
3	1.85	2.17	1.63	0.9
4	2.22	2.09	1.46	1.09
5	1.93	2.43	1.6	0.78
6	1.85	1.99	1.85	0.38
7	1.85	1.48	1.81	0.79
8	1.46	1.65	1.64	1.48
9	1.98	1.64	1.76	0.71
10	1.96	2.18	1.69	0.65
11	2.06	1.67	1.72	0.74
12	2.63	2.44	1.56	0.29
13	2.71	3.18	1.38	0.31
14	1.4	2.41	1.78	0.74
15	1.24	1.44	1.76	1.46
16	1.5	1.69	1.78	1.04
17	1.66	1.86	1.65	1.17
18	1.35	1.71	1.71	1.37
19	0.92	0.95	1.98	1.38
20	0.9	1.02	1.78	1.92
21	1.51	2.13	1.24	1.48

Dark red color highlights average silencing values above 3 (optimal stability). Red color highlights average silencing values above 2 (suboptimal stability). Pink color highlights average silencing values above 1.7 (close to the median stability). Blue color highlights average silencing values below 1 (less than median).