Fig. 1.

Characterization of P. berghei proteins of the FACT complex. A. Schematic representation of proteins of the FACT complex of human, yeast and Plasmodium showing the (location of) conserved domains (see Figs S2 and S3 for the alignment of the gene sequences). The human small unit protein, ssrp1, contains a high-mobility group (hmg) box domain which is absent in the small unit of yeast (Pob3) and Plasmodium and small genes that encode HMG boxes are illustrated for both yeast (nhp6a or b) and Plasmodium (hmg2a or b). B. Northern analyses of transcripts of the large subunit, fact-l and the small subunit facts (left panel) and the two Plasmodium genes encoding HMG-box containing proteins, hmgb1 and hmgb2 (right panel). Transcripts were analysed in synchronized blood stages at different hours post infection (hpi) (5 hpi: ring forms; 16 hpi: mature trophozoites, 20 hpi: immature schizonts; 25 hpi: mature schizonts; gct: purified gametocytes).