Fig. 6.

Δfact-l_gam parasites produce low numbers of oocysts that are blocked in sporogony. A. Although Δfact-l_gam produce low numbers of ookinetes (Fig. 4A), their nuclei show the typically increased fluorescence intensity after Hoechst staining (solid arrows) compared with the haploid ring forms (dashed arrows), indicating normal (DNA replication generating a 4N DNA content) meiosis comparable to wild-type ookinetes. Scale bar = 12 µm. B. Δfact-l_gam parasites produce low numbers of oocysts in A. stephensi mosquitoes that are blocked in early development as shown by the strongly reduced size. None of the oocysts shows nuclear division or sporozoite formation.