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. 2011 Mar;13(3):388–401. doi: 10.1111/j.1462-5822.2010.01541.x

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© 2010 Blackwell Publishing Ltd

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Fig. 5 — Pilin stimulation of the inflammasome is not affected by DNAse and is reduced by pyrococcal protease S. A. BMDMs were treated as indicated with PA103 pilin (250 ng) or plasmid DNA (pUCP20) and DOTAP and/or DNAse I as shown prior to IL-1β measurement in supernatant. Bars are means of three independent determinations; errors bars are ± SEM. Effects of DNAse I treatment were compared with control values by unpaired t-test. For pilin no significant change was observed (n.s. not significant); DNAse significantly reduced the IL-1β released (**P < 0.001). B. Pilin was left untreated or digested with pyrococcal protease S before stimulation of BMDMs in the presence of DOTAP and measurement of released IL-1β. Results are means of three independent determinations; error bars are ± SEM. Untreated pilin + DOTAP produced significantly more IL-1β than DOTAP alone (**P < 0.001, unpaired t-test); proteinase S-treated material was not significantly different from control.