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G, N, and P repletions increase cAMP. Prototrophic yeast (S288C) were starved for G, N, or P and then repleted with the missing nutrient as described in Materials and Methods. Levels of cAMP were measured at the indicated time after repletion using an ELISA assay, with each well receiving extract from the same number of cells (5 OD units). Error bars represent the standard error the mean (n = 3). The experiment was repeated multiple times with similar results.
