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Evolutionary relationship of HLS DUF1220 triplets. Phylogenetic sequence-based alignments of the HLS DUF1220 triplets reveal that the first triplets in each NBPF gene are most similar to one another. Each successive triplet is then most similar to the triplets contained within each NBPF gene, indicating that the hyperamplification of the HLS DUF1220 triplet occurred as the result of intragenic domain amplification.
