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. 2011 Dec 21;441(Pt 2):707–717. doi: 10.1042/BJ20111379

Figure 1. BFA treatment protocols.

Figure 1

(A) Schematic diagram of the secretory pathway components: RER (rough ER), ERGIC, cis-, medial- and trans-Golgi stacks, TGN, p.m. (plasma membrane) and ECM (extracellular matrix). (B) BFA treatment results in Golgi complex disassembly and formation of a fused ER/Golgi compartment. The TGN becomes connected to the recycling endosomal system. (C) A 10-min pulse results in labelled *collagens in the RER and early secretory pathway. After 30 min of chase, these *collagens would be approximately equally distributed between the secretory pathway and ECM (not shown). (D) A 10-min pulse in BFA-treated samples results in *collagens in the fused ER/Golgi compartment. Retained *collagens would not be secreted during the chase (not shown). (E) Continuous labelling floods the secretory pathway and ECM with labelled *collagens. (F) BFA treatment after continuous labelling results in retrieval of *collagens from the Golgi stacks to the intracellular fused ER/Golgi compartment. *collagens in the TGN at the time of BFA treatment are secreted.