Table 2. Effect of collagenase on the relative amounts of intracellular and extracellular *collagens.
The relative amounts of labelled *collagens in the first extracellular salt extract (S1) and in the intracellular detergent extract (N) were quantified by densitometry and expressed as a percentage of 14C *collagen in the extracellular extract. Collagenase treatment significantly decreased the percentage of *collagens in the extracellular extract.
| Extracellular *collagen (%, mean±S.D.) | |||||
|---|---|---|---|---|---|
| Sample | Control | BFA | Control+collagenase | BFA+collagenase | P value (two-way ANOVA) |
| Postnatal CD1 mouse tail tendon | 66.8±5.3 | 61.6±3.0 | 34.2±1.0 | 35.4±7.9 | P<0.01 for collagenase factor (n=3) |
| Postnatal rat tail tendon | 85.6±1.6 | 83.9±4.5 | 64.2±17.7 | 42.4±4.0 | P<0.01 for collagenase factor (n=3) |