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. 2012 Aug 28;446(Pt 3):373–381. doi: 10.1042/BJ20120385

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Cells were either not treated or treated with CPT and stained with DAPI (left-hand panels) and γH2AX antibodies (right-hand panels). Cells were observed after 24 and 48 h of treatment by immunofluorescence microscopy. Scale bar, 10 μm. (b) MDCs were collected, attached to coverslips and stained with DAPI (left) and antibodies against γH2AX (centre) or phospho-Ser¹⁰ histone H3 (right, P-H3) and observed by confocal microscopy. Scale bar, 20 μm.