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. 2012 Aug 29;3:105. doi: 10.3389/fendo.2012.00105

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Copyright © alahpour, Espinoza, Masri, Lam, Barak and Gainetdinov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.

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Bioluminescence resonance energy transfer (BRET) monitoring of β-arrestins recruitment to activated GPCRs. (A) Cartoon of postulated molecular events following activation of a GPCR by agonist that lead to recruitment of arrestin to phosphorylated receptor causing related BRET signal. Arrestins can be tagged with different versions of GFP (YFP, Citrine, or Venus; for review, see Pfleger and Eidne (2006)) and GPCRs are tagged with Rluc to monitor intermolecular interaction by BRET. (B) Titration curves of β-arrestin1–YFP recruitment to D2R–Rluc with or without D2R agonist quinpirole. (C) Titration curves on β-arrestin2–YFP recruitment to D2R–Rluc with or without D2R agonist quinpirole. (D) Dose–response curve of quinpirole effect on β-arrestin2 recruitment to D2R.