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. 2012 Sep;32(18):3743–3755. doi: 10.1128/MCB.00032-12

Fig 1.

Fig 1

Disruption of ndx genes in A. nidulans and introduction of ndxA and sirA alleles to A. nidulans. (A) Strategy for homologous recombination into ndxA, ndxB, and ndxC loci to construct ndxA, ndxB, and ndxC gene disruptants. Total DNA from strains was digested with BstXI (B), SphI (S), and PstI (P) before Southern blotting. Bars indicate positions and sizes of hybridization probes. Lanes 1, 3, and 5, A. nidulans wild type (WT); lane 2, ΔNdxA; lane 4, ΔNdxB; lane 6, ΔNdxC. (B) Strategy for introducing ndxA and sirA to ΔNdxA2 and ΔSirA2. Plasmids harboring wild-type or mutated ndxA and sirA were integrated to chromosomal pyrG regions by single crossover. Total DNA from strains was digested with PstI (P) before Southern blotting. Bars indicate positions and sizes of hybridization probes. Lanes 1 and 5, A. nidulans WT; lane 2, ΔNdxA2; lane 3, ΔNdxA2 plus pBSndxA; lane 4, ΔNdxA2 plus pBSndxA-E57Q; lane 6, ΔSirA2; lane 7, ΔSirA2 plus pBSsirA; lane 8, ΔSirA2 plus pBSsirA-H286N.