Fig 3.

NdxA regulates cellular NAD(H). (A) Transcripts of ndxA, ndxB, and ndxC in A. nidulans wild type (WT) cultured at 30°C and quantified by PCR. (B) Intracellular NAD⁺ hydrolase activity. (C) Time-dependent changes in dry weight of cells. (D) Intracellular NAD⁺. (E) Intracellular NADH. (F and G) Intracellular NAD⁺ hydrolase activity and NAD(H) after a 72-h culture. WT, wild type; ΔndxA, ΔNdxA; ΔndxB, ΔNdxB; ΔndxC, ΔNdxC; ΔndxA+ndxA, ΔNdxA2 strain transformed with pNdxA; ΔndxA+E57Q, ΔNdxA2 strain transformed with pNdxA-E57Q. Data are means ± standard deviations (n = 3). *, P < 0.005; **, P < 0.03 versus WT.