Fig 8.

Immunoprecipitations of GATA-1 and PRC2 components. Western blot results were obtained after immunoprecipitation (IP) with the anti-EZH2, anti-GATA-1, or anti-IgG (isotype-matched immunoglobulin G; mo, mouse; ra, rat). IP were made in tamoxifen (Tmx)-treated G1E-ER4 cells (A), in COS7 cells engineered to ectopically express GATA-1 protein (B), or in tamoxifen (Tmx)-treated G1E-ER4 cells treated with ethidium bromide (EtBr), RNase I, and DNase I (C). Protein interactions were revealed by immunodetection with anti-EZH2, anti-GATA-1, anti-SUZ12, and anti-CKD9. Specific bands are labeled and molecular masses are given on the right and left sides of panels, respectively. The dot indicates a nonspecific band. FH-GATA-1, Flag-HA-GATA-1 fusion protein.