Table 1.
Oligonucleotides used for RT-qPCR analysis
| Specificity (murine cDNA) | Symbol | Direction | Sequence (5′–3′) |
|---|---|---|---|
| Hairy and enhancer of split 1 | Hes1 | F | ACACCGGACAAACCAAAGAC |
| R | ATGCCGGGAGCTATCTTTCT | ||
| Hypoxanthine guanine phosphoribosyl transferase | Hprt | F | CACAGGACTAGAACACCTGC |
| R | GCTGGTGAAAAGGACCTC | ||
| Actin beta | Actb | F | ATCGTGGGCCGCCCTAGGCACCA |
| R | TCCATGTCGTCCCAGTTGGTAACAA | ||
| Hemoglobin alpha adult chain 1 | Hba-a1 | F | AACTTCAAGCTCCTGAGCCA |
| R | GGCAGCTTAACGGTACTTGG | ||
| Hemoglobin beta adult major chain | Hbb-b1 | F | AACGATGGCCTGAATCACTT |
| R | ACGATCATATTGCCCAGGAG | ||
| Aminolevulinic acid synthase 2 | Alas2 | F | GCAGCAGCTATGTTGCTACG |
| R | ACAGGTTGGTCCTTGAGTGG | ||
| Kruppel-like factor 1 | Klf1 | F | ACCACCCTGGGACAGTTTCT |
| R | GAAGGGTCCTCCGATTTCAG | ||
| BCL2-like 1 | Bcl2l1 | F | GGGATGGAGTAAACTGGGGT |
| R | TGCAATCCGACTCACCAATA | ||
| Erythropoietin receptor | Epor | F | TACCTCCCACTCCACCTCAC |
| R | GCTCTGAGTCTGGGACAAGG | ||
| Transcription factor Pu.1 | Pu.1 | F | CACGTCCTCGATACTCCCAT |
| R | GCTGGGGACAAGGTTTGATA | ||
| Transferrin receptor 2 | Trfr2 | F | CTGCCGCTAGACTTCGGCCG |
| R | CGCCGCACGGATGTAGTCCC | ||
| Notch gene homolog 1 | Notch1 | F | TGTTGTGCTCCTGAACAACG |
| R | GTGGGAGACAGAGTGGGTGT |