Table 2.
Oligonucleotides used for ChIP analysis
| Specificity (murine genomic DNA) | Symbol | Direction | Sequence (5′–3′) |
|---|---|---|---|
| Hairy and Enhancer of Split 1 gene promoter (pro1) | Hes1 (pro1) | F | CTCTTCCTCCCATTGGCTGA |
| R | GCACCAGCTCCAGATCCTGT | ||
| Hairy and Enhancer of Split 1 gene promoter (pro2) | Hes1 (pro2) | F | GGAAGTTTCACACGAGCCGT |
| R | GCACCAGCTCCAGATCCTGT | ||
| Hairy and enhancer of split 1 gene open reading frame | Hes1 (ORF) | F | TGGCGGCTTCCAAGTGGTGC |
| R | TGTGAGCGAAGGCCCCGTTG | ||
| Tamm-Horsfall protein (uromodulin) gene promoter | Thp | F | GGTGGATGGTGTGGTCACAAC |
| R | GGTCTTGACACACCAGCTTT | ||
| Pancreatic alpha-amylase promoter | Amy2 | F | TCAGTTGTAATTCTCCTTGTAGGG |
| R | CCTCCCATCTGAAGTATGTGGGTC | ||
| Hypersensitive site 2 from β-globin locus | HS2 | F | CCTTGCCTGTTCCTGCTCA |
| R | CACATGTGACCTGTCTGCCAG | ||
| Hemoglobin beta adult major chain gene promoter | βmaj | F | CAGTGAGTGGCACAGCATCC |
| R | CAGTCAGGTGCACCATGATGT | ||
| Gata2 kb −2.8 enhancer | Gata2 −2.8 | F | GCATGGCCCTGGTAATAGCA |
| R | CAGCCGCACCTTCCCTAA | ||
| Gata2 promoter 1G | Gata2 1G | F | AGATACCCAGAAGGTGCACGTC |
| R | GCAGACCCTGCACCCCT | ||
| Kit oncogene gene promoter | c-kit | F | CACCTCCACCATAAGCCGAAT |
| R | CTCCTAGACAATAAAGGACAACCA | ||
| Notch gene homolog 1 promoter | Notch1 | F | GCATGAGAGGCTGTGTTGACG |
| R | TGGCTGTGTCCGACTTAGGTG |