Fig 5.

IPC-specific dCbl knockdown leads to elevated dilp expression with reduced stress tolerance and longevity. (A) The mRNA abundance of dilp2, dilp3, or dilp5 was assessed by qRT-PCR in the heads of male adult dilp2G4>+ and dilp2G4>dCbl-Ri flies (3 days old, 90/group, n > 3 independent experiments) under the fed or starved state. Normalization was done using RPL32 as an internal control. Data are represented as the mean ± SEM; *, P < 0.05 versus dilp2G4>+ control flies by one-way ANOVA. (B) Photo of male adult dilp2G4>+ (left) and dilp2G4>dCbl-Ri (right) flies. The body weight was measured under fed states (3 days of age, 90 flies/group), shown as the mean ± SEM; *, P < 0.05 by Student's t test. (C) Life span. Survival curves of male dilp2G4>+ (n = 266) and dilp2G4>dCbl-Ri (n = 293) flies. χ² = 68.10; P < 0.0001 by log-rank test. (D) Sensitivity to paraquat. Male adult flies at 3 days of age were treated with 30 mM paraquat, and survival rates were determined (264 dilp2G4>+ flies and 265 dilp2G4>dCbl-Ri flies). χ² = 90.12; P < 0.0001 by log-rank test.