Fig 6.

Metabolic effects of neuronal or IPC-specific suppression of dCbl expression. (A and B) Whole-body glycogen or trehalose levels were measured in male adult flies under fed conditions (3 days of age, 10 flies/group, n > 3 independent experiments). Values for elavG4>+ versus elavG4>dCbl-Ri flies (A) or dilp2G4>+ versus dilp2G4>dCbl-Ri flies (B) were normalized to total protein levels. Data are presented as the mean ± SEM; *, P < 0.05 versus the >+ control group by one-way ANOVA. (C and D) Sensitivity to starvation. Survival curves of starved male adult flies at 3 days of age were determined for elavG4>+ flies (n = 226) versus elavG4>dCbl-Ri flies (n = 205) (C) and dilp2G4>+ flies (n = 137) versus dilp2G4>dCbl-Ri flies (n = 133) (D). χ² = 163.5 (C) and χ² = 29.66 (D); P < 0.0001 versus >+ control group by log-rank test.