Fig 8.

Inhibition of dEGFR signaling reverses the effects of IPC-specific dCbl knockdown without affecting IPCs. (A) The brain IPCs were visualized using GFP in male adult dilp2G4>GFP, dilp2G4>GFP, dCbl-RNAi, and dilp2G4>GFP, dEGFR-DN flies at 3 days of age. Shown are the IPC bodies localized medially in the pars intercerebralis. (B) The body weight of male adult flies was measured under fed states (90 flies/group). (C) The mRNA abundance of dilp2, dilp3, or dilp5 in the head of male adult flies (90 flies/group, n > 3 independent experiments) was determined by qRT-PCR using RPL32 or α-Tub84B as the internal control for normalization. Data in panels B and C are shown as the mean ± SEM; *, P < 0.05 versus dilp2G4>+ control; #, P < 0.05 between dilp2G4>dCbl-RNAi and dilp2G4>dRi, DN by one-way ANOVA.