Fig. 1.
DNA adduct formation (a) and lacZ mutant frequency (b) in the lungs and livers from MutaMouse subchronically exposed to BaP. Levels of dG-N 2-BPDE adducts were determined using the nuclease P1 enrichment version of the 32P-postlabeling method. Data are represented as average ± SEM (n = 5 mice/group). ND, not detected. Average lacZ mutant frequency was determined using the P-Gal positive selection assay. Values shown are average frequencies ×105 ± SEM. Asterisk (*) indicates significance by Student’s t-test (p < 0.01) compared with controls. Transgene mutant frequency data for the liver are from the study by Malik et al. (2012).