Table 5.
Top 10 genes up- or down-regulated by drought in WT and Ljgln2-2.
| WT | |||
|---|---|---|---|
|
| |||
| Probeset | log2 FC | Description | Similar to |
| Up-Regulated | |||
| TC11101 | 4.83 | Glutamate decarboxylase | AT5G17330 |
| chr1.TM1635.18 | 4.77 | ACC synthase | AT3G61510 |
| Ljwgs_047159.1 | 4.61 | STIG1-related protein | AT1G11925 |
| chr5.CM0909.59 | 4.47 | Glutathione S-transferase | AT2G29420 |
| chr1.CM0141.2 | 4.37 | Nitrate/peptide transporter | AT1G32450 |
| Ljwgs_036708.1 | 4.32 | Pectinesterase | AT2G45220 |
| chr4.CM0429.5 | 4.32 | Mitochondrial inner membr. translocase | AT4G16160 |
| chr5.CM0089.120 | 4.15 | Inositol-1,4,5-trisphosphate 5-phosphatase | AT1G47510 |
| chr5.CM0148.50.2 | 4.11 | Cytochrome P450 | AT5G52400 |
| TM0763.11 | 4.08 | 12-oxophytodienoate reductase | AT2G06050 |
| Down-regulated | |||
| Ljwgs_040576.1 | −4.15 | Glutaredoxin | AT5G18600 |
| Ljwgs_056053.1 | −4.15 | Alpha-expansin family protein | AT2G39700 |
| Ljwgs_006332.1 | −3.92 | Hypothetical protein | AT1G30260 |
| chr1.BM1732.18 | −3.82 | Hypothetical protein | AT3G11210 |
| BM0976.11 | −3.78 | Hypothetical protein | AT2G01050 |
|
| |||
| WT | |||
|
| |||
| Ljwgs_028040.1 | −3.74 | Ammonium transporter | AT4G13510 |
| chr1.CM0233.42 | −3.47 | Nucleic acid binding protein | AT1G52950 |
| chr6.TM1374.27 | −3.41 | hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase-like | AT2G19070 |
| Ljwgs_016759.2 | −3.41 | Chloride channel protein | AT5G40890 |
| TM1490.11 | −3.35 | MYB transcription factor | AT2G21650 |
| Ljgln2-2 | |||
|
| |||
| Probeset | log2 FC | Description | Similar to |
|
| |||
| Up-Regulated | |||
| chr1.CM0012.67 | 8.20 | Thaumatin-like protein | AT1G20030 |
| Ljwgs_075692.1.1 | 7.87 | GDSL esterase/lipase | AT1G29660 |
| chr3.TM1465.12 | 7.50 | SPX domain-containing protein | AT2G45130 |
| chr1.TM1635.18 | 7.34 | ACC synthase | AT3G61510 |
| Ljwgs_036708.1 | 6.93 | Pectinesterase | AT2G45220 |
| chr2.CM1150.57 | 6.84 | Metalloendoproteinase | AT1G70170 |
| chr1.CM0104.32 | 6.84 | NAC domain transcription factor | AT3G04070 |
| Ljwgs_020980.2 | 6.80 | C2H2 transcription factor | AT2G37430 |
| Ljwgs_047159.1 | 6.73 | STIG1-related protein | AT1G11925 |
| Ljwgs_145133.1 | 6.72 | Late embryogenesis abundant (LEA) | AT3G53040 |
| Down-regulated | |||
| chr1.BM1732.18 | −8.81 | Lipase/hydrolase protein | AT3G11210 |
| chr2.CM0249.113 | −7.27 | Myb familily transcription factor | AT2G19510 |
| Ljwgs_040576.1 | −6.77 | Glutaredoxin | AT5G18600 |
| Ljwgs_073999.0.1 | −6.70 | Cytochrome P450 | AT1G24180 |
| Ljwgs_052903.1 | −6.12 | Lipoxygenase | AT1G55020 |
| Ljwgs_058749.1 | −5.73 | Beta-glucosidase | AT5G42260 |
| Ljwgs_127990.1 | −5.62 | Myb-related transcription factor | AT4G39250 |
| TM0990.31.1 | −5.59 | Hypothetical protein | - |
| chr3.CM0590.43 | −5.55 | Hypothetical protein | AT4G27450 |
| Ljwgs_091781.1 | −5.45 | Lipoxygenase | AT1G55020 |
The table shows the probesets corresponding to the 10 genes that showed higher extent of modulation (FDR < 0.05) in their transcript levels in the leaves of L. japonicus plants after 4 days of water deprivation as determined by transcriptome analysis using Affymetrix genechips. The log2 of the fold-change is indicated together with the description of the most probable match and the most similar Arabidopsis gene. The following databases were used for the search: the Kazusa DNA research institute (Available online: http://www.kazusa.or.jp/lotus/blast.html; accessed on 16 March 2012), Non-redundant protein sequences (Available online: http://blast.ncbi.nlm.nih.gov/; accessed on 16 March 2012), TAIR (Available online: www.arabidopsis.org; accessed on 16 March 2012) and the Legume transcription factor database (Available online: http://legumetfdb.psc.riken.jp/index.pl; accessed on 16 March 2012).