Table 2.
MICs (/MBCs; μg/mL) and fractional inhibitory concentration indices (FICIs) of Isojacareubin in combination with four conventional antibacterial agents against 10 clinical isolates of SCCmec III type MRSA.
| Agents | ISJa | AMP | CAZ | LEV | AZM | ||||
|---|---|---|---|---|---|---|---|---|---|
|
|
|
|
|
|
|||||
| Alone | Alone | Combb | Alone | Comb | Alone | Comb | Alone | Comb | |
| Ranges of MIC (/MBC) (μg/mL)c | 4(/16)~ 16(/64) | 16(/64)~ 128(/512) | 4~16 + 1~4 | 128(/128)~ 512(/ex) + 32~128 | 32~128 + 1~4 | 4(/8)~ 16(/64) | 1~4 + 1~4 | ex | 512~1024 + 4~16 |
| MIC50c | 8 | 64 | 8 + 4 | 512 | 32 + 1 | 16 | 2 + 2 | - | 1024 + 8 |
| MIC90 | 16 | 128 | 16 + 4 | 512 | 64 + 4 | 16 | 4 + 4 | - | 1024 + 16 |
| Ranges of FICId | - | - | 0.37~0.62 | - | 0.25~0.5 | - | 0.25~0.5 | - | 1~1.5 |
| FICI50 | - | - | 0.37 | - | 0.25 | - | 0.37 | - | 1.5 |
| FICI90 | - | - | 0.62 | - | 0.37 | - | 0.37 | - | 1.5 |
| Effect (%)e | - | - | ad(30) sn(70) | - | sn(100) | - | sn(100) | - | id(100) |
a
ISJ, Isojacareubin; AMP, Ampicillin; CAZ, Ceftazidime; LEV, Levofloxacin; AZM, Azithromycin;
b
Comb, combined (the binate data are expressed as values of single antibacterial + ISJ);
c
MIC50, concentration of inhibition against 50% of MRSA strains; MIC90, concentration of inhibition against 90% of MRSA strains; ex, concentration of >1024 μg/mL;
d
FICI50, FICI of inhibition against 50% MRSA strains; FICI90, FICI of inhibition against 90% MRSA strains;
e
ad, additivity; id, indifference; sn, synergy.