Table 1.
Repeatability, reproducibility and accuracy of HPLC method used for aflatoxin determination.
| Aflatoxin | LOD a (ng g−1) | LOQ b (ng g−1) | Calibration Curve | R2 | Recovery (%) c | Mean (μg kg−1) ± RSD (%) d |
|---|---|---|---|---|---|---|
| AFB1 | 0.02 | 0.05 | y = 68,983x + 34,942 | 0.9997 | 97.6 | 125.3 ± 9.12 |
| AFB2 | 0.01 | 0.02 | y = 104,767x – 6,094 | 0.9995 | 91.2 | 15.3 ± 2.01 |
| AFG1 | 0.02 | 0.05 | y = 32,045x + 2,780 | 0.9996 | 97.6 | 15.3 ± 1.44 |
| AFG2 | 0.01 | 0.02 | y = 61,801x − 85.618 | 0.9991 | 91.2 | 6.3 ± 3.42 |
a
Limit of detection;
b
Limit of quantification;
c
Accuracy was determined by the determination of the recoveries of aflatoxins. By spiking 125.5 μg kg−1 aflatoxin B1, 15.3 μg kg−1 aflatoxin G1 and B2 and 6.3 μg kg−1 G2 to the samples (uninfected ground and tree nuts);
d
Replicate analysis of each spiked sample was used to determine the accuracy, expressed as mean (μg kg−1) ± relative standard deviation (%).