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PCR banding profiles of wheat (W), rye (R), triticale (T) and seven wheat-rye addition lines (numbers correspond to rye homologous chromosomes pair) obtained with primers designed to amplify the three internal segments of pSc20H: 20H1, 20H2 and 20H3. All the pSc20H internal primers amplified a band with the expected size and identical in wheat, rye and triticale. Segment 20H1 is also amplified in all wheat-rye addition lines. Molecular weight marker: 1 Kb Plus DNA Ladder.
