Table 1.
Characterization of PCR banding profiles obtained with OPH20 10-mer primer in wheat, rye, triticale and wheat lines with the addition of rye chromosomes.
| Number of bands in distinct genotypes profiles | |||||
|---|---|---|---|---|---|
|
| |||||
| Parental lines | Hybrid lines | ||||
|
|
|
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| Triticale | Addition lines | ||||
|
|
|
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| Wheat | Rye | Expected | Observed | Wheat + 1R, 5R, 6R | Wheat + 2R, 3R, 4R, 7R |
| 2 | 2 | 4 | 3 | 3 | 2 |
| OPH20Wheat850 | pSc20H | pSc20H | pSc20H | OPH20Wheat85 | |
| OPH20Wheat650 | OPH20Rye1300 | OPH20Rye1300 | OPH20Wheat850 | OPH20Wheat650 | |
| OPH20Wheat650 | OPH20Wheat650 | ||||
|
| |||||
| Parental origin | |||||
| Wheat | 2 | 1 | 2 | 2 | |
| Rye | 2 | 2 | 1 | 0 | |
Note: In triticale restructuring events are revealed by the difference between the number of observed bands and the number of expected ones (corresponding to the sum of the number of bands detected in parental lines). In wheat-rye addition lines, two groups presenting distinct banding profiles are characterized.