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. 2012 Jul 10;13(7):8562–8577. doi: 10.3390/ijms13078562

Figure 3.

Figure 3

Effect of FWFE on TNF-α-induced inhibition of insulin signals in FL83B cells. FL83B cells were incubated in serum-free F12K medium, with or without added TNF-α (20 ng/mL), incubated at 37 °C for 5 h, transferred to another serum-free F12K medium with or without insulin (1000 nM), FWFE (6.25 μg/mL), and then incubated for an additional 30 min. The basal level of glucose uptake was evaluated by incubating cells with the serum-free F12K medium. The relative expressions of IR, IR-tyr, IRS-1, PI3K (p85), p-Akt/Akt, and GLUT-2 in each treatment group were calculated using actin as the standard. Letters a~d indicate significant differences at 5% level.