Table 3.
Primer | Primer sequence | Primer couple | Product (bp) | Annealing temperature |
---|---|---|---|---|
APF1 | AGCAGGCAACGCGGTAAAA | APR1 APR3 |
548 041 |
58 58 |
APR1 | GCCTTCTTGATCATGTTCCTCCTC | APF1 | 540 | 58 |
APF2 | GGATCCATGCCGAAGCGTGGGAAAA | APR2 | 854 | 58 |
APR2 | AGTAATCAAGGCGCCAACCAACAT | APF2 | 854 | 58 |
APR3 | TTGGGGAAAGGTCACAGT | APF1 APF2 |
1041 954 |
55 52 |
AP1qF | GGTAAAGGAGGAAGCCCCAGATA | AP1qR | 194 | 56 |
AP1qR | TCACCAATGCCATAGGAGACTTT | AP1qF | 194 | 56 |