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. 2012 Jul 20;13(7):9110–9128. doi: 10.3390/ijms13079110

Figure 4.

Figure 4

Figure 4

Expression of RPW8.1 and RPW8.2 genes from Arabidopsis thaliana ecotype Ms-0 in interaction with Trichoderma atroviride. (A) Development of interaction of Arabidopsis thaliana ecotype Ms-0 plantlets with Trichoderma atroviride. (a), (b), (c) and (d) photographs of 15-day-old Arabidopsis (Ms-0) plantlets inoculated with T. atroviride, the plantlets were photographed at (a) 48 h, (b) 72 h, (c) 96 h and (d) 120 h post-inoculation. (e), (f), (g) and (h), photographs of 15-day-old Arabidopsis (Ms-0) control non-inoculated plantlets of (e) 48 h, (f) 72 h, (g) 96 h and (h) 120 h; (B) Induction of RPW8.1 and RPW8.2 genes by T. atroviride interaction. Quantification of RPW8.1 and RPW8.2 genes by qRT-PCR, expressed as relative mRNA level compared with a control conditions, was calculated after normalization to the Arabidopsis actin 8 gene using the comparative threshold method. Analyses were performed by triplicate. Control conditions or non-inoculated plantlets (light grey bars) and tester conditions or inoculated plantlets (dark bars) at 48, 72, 96 and 120 hpi, respectively.